Crosslinking mass spectrometry (CLMS) has become a standard addition to cryo-EM structures helping to assign proteins to densities and place dynamic regions. It is also replacing classical truncation analyses in molecular biology labs for identifying interaction regions.

Intriguingly, any proteomics facility can conduct crosslinking analyses with a moderate adjustment to already established procedures. Getting some sort of data is in fact rather straight forward. However, results can be improved very substantially through experience (own or taught). Changes to sample preparation and mass spectrometric acquisition are minor but critical. Large care must be taken while mastering the data analysis workflow.

Being among the pioneers of this technology we hold a detailed understanding of the entire workflow and have collaborated with numerous structural and molecular biology labs. Now, we would like to pass on our experience to core facilities in a dedicated workshop to help spread the technology. In this workshop, we will provide guidance for successful setup and application of CLMS and share our twenty years hands-on experience in CLMS.

We hope that this will also generate a network of interested facilities that will carry beyond the workshop and help addressing any implementation or unforeseen issues encountered with CLMS in the future.

• State-of-the-art developments in crosslinking mass spectrometry
• Experimental design
• Instrument setup
• Software tools
• Data handling
• Effective project communication
• Hands-on experience sharing
• “Bring your own data” workshop

Apply by submitting the application form (pdf) together with a list of LC-MS instruments available to your facilities and a 0.5 – 1 page motivation letter detailing what you would like to achieve with the course and how the course would help you in your facility work to clmsrappsilber@bioanalytik.tu-berlin.de

Confirmation of successful registration will be send out by 15th April 2020

Registration Fee: 1000 Euro

Registration fee includes:

• Registration
• Course materials
• Coffee breaks with light beverages and biscuits

Note: Travel, food and accommodation are not included in the registration fee.

Download program (pdf)

clmsrappsilber@bioanalytik.tu-berlin.de